ecto nucleotidase cd73 inhibitor  (MedChemExpress)

Journal: Purinergic Signalling

Article Title: Adenosine receptors regulate exosome production

doi: 10.1007/s11302-020-09700-7

Figure Lengend Snippet: Chemicals used in this study (chemicals used in this study and information about the used concentrations and sources)

Article Snippet: Ecto-5′-nucleotidase (CD73) inhibitor , Adenosine 5′-(α,β-methylene)diphosphate sodium salt (AMPCP) , As indicated , Tocris.

Techniques: Concentration Assay, Phospho-proteomics

Journal: Purinergic Signalling

Article Title: Adenosine receptors regulate exosome production

doi: 10.1007/s11302-020-09700-7

Figure Lengend Snippet: ADOR signalling regulates exosome production in head and neck cancer cells. a Gene expression of A1R, A2AR, and A2BR of UMSCC47 cells. b Western blots of isolated exosomes derived from UMSCC47 cells treated with or without the non-selective ADOR agonist CADO. TSG101 and CD9 antibodies were used as exosome markers and a Grp94 antibody served as a negative control. c TEM images of isolated and negatively stained UMSCC47-derived exosomes. Cells were cultured in the presence or absence of CADO. d Size distributions of UMSCC47-derived exosomes were measured by qNano. e–h Levels of total exosome protein in μg normalized to 106 cells derived from UMSCC47 cells in the presence of indicated concentrations of CADO (e), the non-selective ADOR antagonist CGS-15943 (f), and the ecto-5′-nucleotidase (CD73) inhibitor AMPCP (g) and in the presence of the non-selective P2 agonist α,β-meATP (h). Values represent means ± SEM of three independent experiments; p values indicate differences compared with CTRL and were assessed by repeated measures ANOVA

Article Snippet: Ecto-5′-nucleotidase (CD73) inhibitor , Adenosine 5′-(α,β-methylene)diphosphate sodium salt (AMPCP) , As indicated , Tocris.

Techniques: Gene Expression, Western Blot, Isolation, Derivative Assay, Negative Control, Staining, Cell Culture